Red Reflex

Issues of Concern

If pupils are not large enough to allow light to enter into the eye, this will result in an absent red reflex. Performing the examination in a darkened room and starting with the smallest diameter beam of light setting on the ophthalmoscope are two techniques that can be used to help in this condition. Topical medications to dilate the pupil, such as tropicamide, cyclopentolate hydrochloride, and phenylephrine, can also be used.[6] There are specific, suggested dosages for children of different ages.

The validity of the red reflex examination has been the object of extensive study in the literature with varying results.[7][8][9][10][11] Overall, the Brückner test has shown to be a sensitive and specific marker for amblyopia, anterior and posterior segment opacities, and strabismus in children. Results do vary with different equipment as well as the expertise of the examiner and may result in a high number of false negatives leading to unnecessary referrals. 

Clinical Significance

The purpose of vision screening is to identify patients with ocular abnormalities that may require urgent referral to an ophthalmologist for potential sight and sometimes life-saving treatment. Vision system screening in neonates, infants, and children includes several components and should occur during each well-child visit. Vision screening should begin with the detection of risk factors requiring a thorough review of family and personal ocular history. Premature birth, cerebral palsy, down syndrome as well as a family history of strabismus, retinoblastoma, congenital cataracts, or genetic diseases are critical risk factors to consider.

The eye examination should evaluate the following: external structures of the eyes, including the eyelids, motility, eye muscle balance, pupils, and red reflex. The red reflex test can be performed relatively quickly as well as noninvasively, which is useful for evaluating an apprehensive child. This test has shown to have high sensitivity for testing abnormalities in both the anterior segment and posterior segments of the eye, and a normal red reflex can rule out intraocular pathology. In contrast, an abnormal reflex indicates the need for an ophthalmologic referral for comprehensive evaluation.

To be considered normal, a red reflex should be seen in both eyes symmetrically and equivalent in color, intensity, and clarity. An absent or black reflex may indicate an obstruction that is preventing light from reflecting back to the examiner. An absent red reflex can result from cataracts, corneal scars, or vitreous hemorrhage.[12] Debris over the surface of the eye may also cause a black reflex, so the examiner should ask the patient to blink and check for the red reflex again. Examiners should be aware of the routine topical application of the prophylaxis erythromycin eye ointment and try to avoid examining the red reflex within approximate time to the ointment application.

Abnormalities associated with abnormal red reflex can be grouped into two categories;

The first one is leukokoria ( white reflex), which can result from the following:  

• Retinoblastoma: a neuroblastic tumor arising from immature cells of the retina and occurs in one of 20000 children, making it one of the most common malignancies of childhood.[13] Other signs and symptoms of retinoblastoma include red eye, irritation, glaucoma, strabismus, and delayed growth development. Retinoblastoma, if detected early, has a 90% cure rate with proper management; however, it is fatal without treatment. Another cause of leukokoria is a cataract, which is clouding of the lens of the eye. Pediatric cataracts can be congenital or acquired.[14] Cataracts can occur unilaterally or bilaterally, leading to blurry vision or permanent vision loss.
• Pediatric cataracts may have a genetic disposition or may occur spontaneously.[15] In adults, cataracts often develop slowly, and disease progression can be monitored until clinical visual disturbances present. However, in the developing eyes of children, the brain is continually making neuronal connections for vision development. Congenital cataracts can obstruct this process and lead to serious long-term effects such as amblyopia or uncorrectable vision loss. Early detection and management of cataracts in the pediatric population can prevent blindness. Assessment of the red reflex is necessary on every neonate before leaving the hospital, as most congenital cataracts are detected in that period. Treatment includes surgery to remove the cataract.
• Glaucoma
• Retinal detachment
• Retinopathy of prematurity
• Posterior coloboma,
• Other optic nerve abnormalities.

The second group includes conditions that result in an abnormal pattern of red reflexes, such as:

• Refractive errors ( hyperopia, myopia, or astigmatism): can alter the red reflex, creating an abnormally asymmetric and nonhomogeneous red reflex. The shade of reflected asymmetry that is visible through the aperture of the ophthalmoscope can help to identify the problem.[16] As light enters through the eye, it will get refracted by the optics of the eye. For instance, in patients with myopia, a peripheral crescent of light will appear on the same side of the light source, whereas in patients with hyperopia, the peripheral crescent of light will appear on the side opposite of the light source. A normal red reflex in these patients is only present if the patients wear their corrective glasses or contact lenses.
• Abnormally sized, shaped, or positioned red reflex can result from aniridia (a condition in which there is no iris), coloboma (a hole in the iris or other structures of the eye), dilating drops, and trauma.[17]

Nursing, Allied Health, and Interprofessional Team Interventions

The American Academy of Pediatrics and American Academy of Ophthalmology recommends screening eye examinations for all newborns as well as children at specific intervals of age during office visits. The eye exam is an essential part of assessing neonates because the prognosis of ocular disorders in children is highly dependent on early detection and referral.[18] [Level 5] The neonatal eye screen involves the use of an ophthalmoscope to check the red reflex as well as a careful observation of anatomic structures and visual behavior. If undetected, ocular disorders in children can result in permanent vision loss and, rarely, death. Early screening is also crucial for the critical period of development in neonates and infants. A vision screening can occur in the pediatrician or primary care office, in the school nurse office, or even in the emergency department.[19] Implementing a standard way of screening vision in children in the primary care setting has demonstrated to be the most effective method of detecting vision problems promptly.[20] It is essential to follow up with children who get referred to make sure a pediatric ophthalmologist has adequately evaluated them. 

There are validated instrument-based screening devices available that images the red reflex to detect a refractive error, amblyopia, and strabismus in children without pharmacological pupillary dilation.[21][22][21] These automatic instruments, such as the photo-screeners and autorefractors, are easy-to-use and can be implemented in the primary care setting as a part of reliable and sensitive vision screening.