Artificial Cornea Transplantation

Anatomy and Physiology

Classification

1. Collar-stud keratoprosthesis with 2 skirt plates, e.g., Boston KPro, Auralab KPro, which has donor cornea between the plates.
2. Soft optic and skirt made from polymers with an intracorneal securing of the skirt within the stromal layers, e.g., Legeais, AlphaCor, KeraKlear.
3. The keratoprosthesis device is secured on the external cornea and sclera, with a central clear optical core, e.g., osteo-odonto-KPro (OOKP). 

Boston KPro Type I

Early models of the Boston KPro had a solid backplate, resulting in higher rates of cornea tissue melt with follow-up. This was due to reduced aqueous flow and penetration to the donor carrier cornea tissue and delivery of nutrients, leading to tissue necrosis and device extrusion or leakage and endophthalmitis. Therefore the backplate was modified to have holes, and a bandage contact lens changed at regular intervals to avoid ocular surface desiccation with long-term prophylactic topical vancomycin administration. This increased biointegration of the KPro with the donor cornea, reducing rates of leakage of pathogenic microbes into the eye.  

Osteo-odonto-Keratoprosthesis (OOKP)

Previously, porous and nonbiological KPro skirt materials have been trialed, including Teflon, ceramic, Dacron, PTFE, hydrogel, and hydroxyapatite.[22] All of these had issues with longevity and tissue integration. The osteo-odonto-keratoprosthesis (OOKP) uses a tooth root-alveolar bone complex, originally described by Strampelli, as a biological substitute for the KPro skirt. Nowadays, tibial bone is sometimes used as an alternative in patients with no teeth (edentulism). The modern-day OOKP is the modified type improved by Falcinelli et al. and standardized in the Rome-Vienna protocol.[23] The patient's ocular surface is bypassed with a buccal mucosal membrane (BMM) graft, and the anterior segment structures are replaced with an osteo-odonto-acrylic lamina. The BMM can tolerate drying and some inflammation, and the alveo-dental lamina integrates with the ocular tissues and can last for several decades. The surgery is done in stages by oral and ophthalmic surgeons.

Indications

Patients with severe corneal opacity that are bilaterally blind where otherwise conventional corneal and stem cell transplantation would fail can be considered for artificial corneal transplant in one eye if that eye has a reasonable retina optic disc. The degree of ocular surface scarring or keratinization and inflammation, any shortening of the fornices, baseline tear secretion, blink reflex, and lid abnormalities will need to be assessed. These will influence the surgical approach and choice of the keratoprosthesis.

Boston KPro Type I 

For KPro Type I patients need to have an adequate blink reflex and tear production with a minimum Schirmer 1 out of 5 mm test. Any type of anterior segment pathology can be acceptable. However, the severity and type of posterior segment disease will impact suitability and clinical outcomes. Examples would include previous multiple failed grafts, corneal opacities with extensive vascularisation, certain conditions such as aniridia, corneal dystrophies, herpetic keratitis, previous corneal infections. The patient will need to apply and retain a soft contact lens if the type I KPro is being considered.[20] Boston Type II KPro can be suitable for patients who do not fit these criteria and who are also not suitable for OOKP due to poor dentition or edentulism.

OOKP

Patients with dry eyes, defective blinking and lid abnormalities, keratinization and inflammation of the ocular surface such as Sjogren's syndrome, graft versus host disease, Steven Johnson Syndrome, mucous membrane pemphigoid (stage 3 and 4), chemical burns, Lyell syndrome, and trachoma (stage C0 WHO classification) with at least light perception vision may be suitable for OOKP surgery.

Contraindications

Boston KPro Type I

The absence of a blink reflex, tear production, posterior segment pathologies such as retinal detachment, vision worse than light perception, abnormal lid anatomy, and forniceal shortening with the inability to retain a soft contact lens e.g. from forniceal shortening are all contraindications. Patients with no light perception vision, phthisis bulbi, or who have unrealistic expectations are not suitable for either type I or type II KPros. Patients with lagophthalmos require an oculoplastic consultation to minimize exposure risks.

Boston KPro implantation has been previously performed in the pediatric age group due to the perceived advantages over penetrating keratoplasty of reduced rejection, rapid visual recovery (lack of postoperative suture-related astigmatism), fewer examinations under anesthesia. However, recently published experience demonstrated high complication rates and progression to phthisis, hence it is no longer recommended.[24][25]

OOKP

Patients with advanced glaucoma will be a relative contraindication.[3] It is contraindicated in patients under 18 years of age due to their high bone turnover and also in eyes with no light perception vision or phthisis bulbi. Patients with the detached retina or other posterior segment pathology which results in low visual potential are contraindicated for an OOKP. Other relative contraindications include patients who will not be able to complete the follow-up program or who have poor mental health.

Equipment

Boston KPro Type I

Boston KPro type 1, cornea donor tissue, trephine, 9/10.0 nylon sutures, soft bandage contact lens, intraocular lens (if combining with lens removal), corneal graft surgical tray, Oculoplastic surgical tray, vitrectomy tray, and glaucoma drainage device if doing concurrent surgery.

OOKP

Corneal graft surgical tray, cautery, bone saw retractors, diamond-coated flywheel, drill, optic cylinder, fliering ring, trephine, silk traction suture, 10.0 nylon sutures.

Personnel

Ophthalmologists (including subspecialists in cornea and anterior segment, glaucoma, vitreoretinal, oculoplastics), oro-dental surgeons, anesthetists, physicians, nurses, and clinical psychologists are essential for optimum outcomes. Psychological, social support, and provisions for access to hospital facilities in planned and emergency follow-up care are necessary components of the service. The care pathway should also have access to patient support groups, the information in the form of leaflets or multimedia to instruct and educate the patient about the procedure and aftercare.

Preparation

Boston KPro Type 1

Generally, patients will have had maximal medical and surgical management already, and KPro Type 1 surgery is the only option available for vision preservation and improvement. Baseline best-corrected visual function should be documented, including evidence of glaucoma surgeries, previous corneal grafts, history of steroid response, and posterior segment disease. Biometry should be undertaken if open sky cataract extraction will be performed. Risk factors such as herpetic disease, active ocular surface inflammation will need to be optimized before surgery.

OOKP

During the preoperative assessment, baseline visual acuity of at least light perception in the eye is necessary to proceed to OOKP. Ultrasonography, A-scan biometry, and electrodiagnostics also help to predict visual potential postoperatively. Intraocular pressure can be challenging to measure and sometimes may need to be recorded subjectively via digital estimation. A thorough ocular surface examination and documentation of dryness, as well as the assessment of the patient's oral health, is needed. Sometimes radiological imaging of the patient's teeth is necessary to choose a suitable donor tooth.

The patient is started on antiseptic and antifungal mouthwash a day before stage I surgery. During this stage, a BMM is harvested from the patient and grafted to the ocular surface bed, and the prepared osteo-odonto-acrylic lamina is implanted into a subcutaneous pouch in the contralateral lower lid. Preparation of the second stage involves careful monitoring for mucosal complications (thinning, ulceration, infection) and infection or extrusion of the implanted lamina.

Technique or Treatment

Boston KPro Type I

1. Remove thickened corneal epithelium to aid visualization.
2. Mark the center of the cornea with gentian violet. An RK marker can be used to aid suture placement later.
3. If doing concurrent pars plana vitrectomy, the ports can be pre-placed. A core anterior vitrectomy is generally done in aphakic patients.
4. In phakic patients, lens extraction is done as well, with or without implantation of an intraocular lens.
5. KPro is assembled before the trephination of the patient's cornea. The donor cornea is trephined to measure 8.5-9.0 mm. A central 3 mm hole is trephine on the donor corneal button. This can be done before the outer diameter punch is used. The front plate is placed face down. The donor button is placed over the front plate stem, with the backplate placed over the stem resting on the cornea endothelium. This is secured in place with the titanium locking ring.
6. A paracentesis is made, and viscoelastic injected into the anterior chamber. The patient's recipient cornea is trephinated. Usually, the diameter is 0.5 mm less than the donor cornea graft. At this point, any additional procedures are usually undertaken due to better visualization, e.g., lens extraction, implantation/removal of the intraocular lens, open-sky anterior vitrectomy, trimming of glaucoma drainage devices.
7. The donor button with KPro is sutured with interrupted 10.0 nylon (9.0 nylon can also be used) and checked for integrity.
8. A pars plana core vitrectomy (PPV) is sometimes performed after suturing is complete, especially if there is posterior segment pathology (hemorrhage, retinal detachment, epiretinal membrane). Long-term patients who have had combined PPV and KPro had fewer complications.[26]
9. Intracameral antibiotics and a soft contact lens are placed at the end of the procedure.

OOKP[23]

Stage I

1. The mucous membrane covering needs to be harvested. A preoperative oral hygiene regime is started after the decision to operate is taken. The size of the removed MMG is around 3 to 4 mm diameter to cover the anterior surface of the globe to the recti muscles' insertions. The parotid duct opening is spared, along with hemostasis and sutured closure of the graft site.
2. All remaining limbal stem cells, corneal epithelium, and Bowman's layer covering the globe require removal. The conjunctiva is recessed up to the recti muscles with the use of minimal cautery. Tenons capsule is used to cover up a non-vascularised cornea.
3. The MMG is sutured onto the episclera with interrupted or running suture close to the recti insertions without overstretching.
4. The tooth chosen should have the largest root. The gingiva is dissected, and the tooth with root and surrounding alveolar bone is harvested with a bone saw. The ideal size of the dentoalveolar lamina is 15 to 16 mm in length and at least 3mm in thickness. The gingiva is removed and extensive rinsing with povidone-iodine to reduce bacterial contamination.
5. A diamond-coated flywheel is used to remove half of the root. All tissues from the dental pulp canal are also removed.
6. An opening for the optic cylinder is drilled in the dentine at a perpendicular angle, ideally centered or where there is more bone tissue. 
7. The optic cylinder power is calculated (50 to 60 diopters for an aphakic eye) and cemented into the opening after the dentine is dried with oxygen.
8. The tooth crown is removed, and the osteoodontoacrylic lamina (OOAL) implant is inserted into a subcutaneous pouch for 3 months to aid revascularisation and growth of connective tissues, usually in the orbitozygomatic area inferior to the lower lid of the contralateral eye.

Stage II

1. The OOAL is explanted from the subcutaneous pouch and assessed for integrity and bone absorption.
2. The connective tissue is removed from the dentine surface, which will make contact with the corneal surface.
3. The MMG is partially lifted off the anterior globe and a flap fashioned, with the inferior limbus base attached to retain the blood supply.
4. A Flieringa ring is sutured to the sclera with traction sutures at 3 and 9 o'clock pre-placed to lift the ring at the time of lamina insertion.
5. The center of the cornea is trephined to the same diameter as the posterior optical cylinder. Decentration here can cause a visual field decentration.
6. Removal of the iris, lens, and anterior vitreous is necessary to avoid postoperative secondary glaucoma or severe inflammatory membranes.
7. Pre-placed sutures around the prosthesis are done before inserting the optical cylinder into the cornea, with the dentine surface facing the cornea. Air is injected through a 30G needle into the globe for inflation. Interrupted sutures fix the prosthesis to the corneoscleral surface.
8. More sterile air is injected to seal the cornea trephination. The patient should posture on their back for 4 to 5 days after the operation.
9. The buccal mucosa flap is replaced to cover the lamina after a central trephination to allow the optical cylinder to be exposed.
10. Lifelong topical antibiotic therapy and aseptic sterile cleaning of the optical cylinder are advised. Eye ointment should be used if there is lagophthalmos present. A scleral shield can be worn to reduce dehydration and for improved cosmesis.

Complications

Complications can arise from failure of the KPro to biocolonise (non-epithelization) and biointegrate (corneal melt around the optic). Ongoing inflammation in the anterior chamber and the formation of retroprosthetic membranes are common complications. Corneal tissue melt can lead to leakage and endophthalmitis, repaired with the regrafting cornea and autologous cartilage tissue.[27] Higher-risk eyes are those with underlying autoimmunity. Endophthalmitis has been reported to range in the United States between 1 to 12.5% and up to 17% internationally for the Boston keratoprosthesis.[28] This can lead to complete loss of vision.

After successful artificial cornea transplantation (all types), glaucoma is the most common complication and can even affect the eyes during late follow-up. High preoperative intraocular pressure, autoimmune diseases such as mucous membrane pemphigoid, and Steven Johnson syndrome lead to a higher risk for glaucoma development and progression. In a recent single-center study of 140 eyes following Boston KPro Type I implantation, 24% of eyes developed de novo glaucoma postoperatively.[29] Long-term monitoring for glaucoma development and progression is challenging due to the gross anatomical changes. Anterior segment optical coherence tomography can detect changes associated with glaucoma, i.e., angle narrowing, and if the patient has reasonable vision, then standard visual field testing can still occur.[30][31] Several studies have established that implantation of glaucoma drainage devices before or at the time of KPro surgery will reduce the risk of glaucoma progression.[32][33]

Combined KPro-vitrectomy eyes show a decreased incidence of retroprosthetic membrane formation and more stable long-term vision compared with non-vitrectomised eyes.[34] Incidences of cornea melt, endophthalmitis, glaucoma needing surgical intervention increase with follow-up time, and there is a paucity of medium to long-term published data.[35] Complete repeat KPro appears to offer a reduced risk of recurrent melt for the Boston KPro, with localized repair a temporalizing measure and is the only option in cases of device extrusion or severe infection.[36] 

The OOKP is fully biocompatible (if using the patient's tooth); however, complications persist. These range from oral (buccal mucosa harvesting), oculoplastic, secondary glaucoma, posterior segment, and device extrusion. Vitreoretinal complications include retinal detachment, vitritis, retroprosthetic membrane, vitreous hemorrhage, choroidal detachment, and endophthalmitis.[37] Primary vitrectomy at the time of OOKP surgery is sometimes undertaken to reduce these complications. Mucosal melt and ulceration can lead to exposure of the laminar which will require repair with mucosal grafting. Poor oral health and buccal mucosa scarring are risks for mucosal ulcers and thinning. Laminar resorption can occur with anatomical failure and is more pronounced in allografts. Detection is via clinical examination with a CT scan.[38] Steven Johnson syndrome patients are at increased risk of laminar resorption.[39]

Clinical Significance

Corneal opacities are one of the leading causes of worldwide blindness along with cataracts, glaucoma, and macular degeneration. Access to corneal transplantation is variable around the world and is limited by a lack of infrastructure as well as donor tissue supply.[40][41][42] Improvements and advances in cornea keratoprosthesis have enabled more stable, biocompatible devices to be available. To date, there is no equivalence between conventional corneal and artificial cornea keratoprosthesis transplantation. They remain a viable option for patients where conventional cornea transplantation is not possible in the context of scarring and vascularisation.[41] 

The increasing number of newer artificial corneal keratoprostheses require adequate study and evaluation. Recent guidelines on consensus with reporting standards amongst all the groups researching artificial cornea devices have been created and will result in better data collection and comparative analysis.[43]

Enhancing Healthcare Team Outcomes

Artificial cornea transplantation surgery with keratoprostheses is a very complex, multi-stage procedure, and expertise, multidisciplinary teams, and resources are necessary. The ophthalmologist is the first point of patient assessment. The patient's suitability for surgery, risk factors that can influence outcomes, and information is provided to the patient and their family. If assessing for suitability for OOKP, an oral surgeon will be consulted as well. A physician can assess the patient's general systemic health. A clinical psychologist can investigate for psychological morbidity, patient's coping mechanisms, expectations, adaptation to blindness, and availability of social support in place. Opthalmology specialty nurses can assist with the procedure and post-operative care. This type of interprofessional team coordination will bring about improved results from this procedure. [Level 5]